Semen Analysis

SEMEN ANALYSIS

Indications

Infertility

To check the completeness of vasectomy

Medico legal purposes

Macroscopic Examination (Normal Constituents) 

Volume – 4-5ml

Viscosity – high

Color – cloudy grey white

pH – 7.2-8.9

Microscopic Examination

Procedure is done after liquefaction, i.e. 15-30 minutes

Motility place a drop of liquefied semen on the glass slide and cover with a cover slip. Normally 80% sperms are motile.

<50% motility of sperms- significant

Sperm count- draw semen upto 0.5mark in WBC pipette and diluting fluid upto 11 mark (dilution is 1:20). Mix well.

Diluting fluid- components:

Sodium bicarbonate - 5gms

Phenol – 1ml

Distilled water – 100 ml

Charge the counting chamber (Neubauer) and count the cells in four corner squares.

Sperm count/ml = N x 10 x 20 x 1000 / 4

Normal sperm count = 60 to  150 million / ml

Oligozoospermia = <60million / ml