Cerebral Spinal Fluid  Analysis

Cerebral Spinal Fluid(CSF) ANALYSIS:

CSF is formed from the choroid plexus

Technique Lumbar puncture

Site L3L4(adults)

L4L5(children)

Procedure: CSF is often examined in the counting chamber (Neubauer’s chamber) without addition of any diluting fluid. The number of cells are counted in all the 9 large squares. Cell count should be done within 30 min of collection of specimens.

Calculation

No: of cells counted = N

Dilution = 1:1

Depth = 1/10 mm

Area = 9mm2

Cells/liter(mm) = n x 1 x 10 /9

= 1.11 x N

Indications Of Lumbar Puncture

• 1. Diagnostic: Acute Meningitis

     1. 1. 1. SAH

           2. Encephalitis

           3. Brain Abscess

           4. Multiple Sclerosis

           5. Neurosyphilis

           6. Tumors Of Brain And Spinal Cord

  2. Therapeutic: 

     1. 1. 1. Anesthetic Administration

           2. Intrathecal Drug Administration

  3. Contraindications: 

     1. 1. 1. Raised Intracranial Tension

           2. Bleeding Disorder

           3. Kyphoscoliosis